We measure several hundreds of metabolites using absolute quantification, utilizing stable isotope dilution, 6-8 pt standard curves and 4 levels of QC analysis within each batch.  Most quantitative assays are run on a triple quadrupole LC-MS/MS system (API-4500, AB Sciex).  Panels include:

 
 
 
 


 
Intracellular energy metabolite panel: Primary metabolites and cofactors associated with energy production in the cell.  Absolute quantification of nucleotides (e.g. ATP, ADP, AMP, GTP, GDP,...etc), sugar phosphates (glycolysis, pentose phosphate intermediates), coenzymes/cofactors (e.g. NADH, NAD+, acetyl-CoA, etc).  This platform uses either cell culture or tissue as the matrix for analysis (Compound list).
 
 
 
 
 
 
 
 
 
 
 
 
 
Lipidomics Panel:  Phospholipids, fatty acids (saturated, mono- and poly- unsaturated), eicosanoids, acylcarnitines, and bile acids.  Relative quantification of a variety of hydrophobic metabolite classes critical for metabolism, modulating inflammation, and signaling. Peaks are normalized to appropriate stable isotope internal standards for ideal inter-sample comparisons. Method was developed for plasma and serum samples, but is adaptable to all types of biological tissues that contain measurable levels of these metabolites (Compound list). Lipid compound list can be customized for individual projects.


Global metabolite panel: (ideal for plasma, serum, urine, or even cells).  These several hundred metabolites are found both intra- and extracellularly and include amino acids, organic acids, acylcarnitines, purines and pyrimidines, bile acids and more, providing a diverse metabolic profile.  The full method uses two separate runs to cover multiple ionization modes (Compound list).