Metabolomics / Biomarker discovery
Biomarker / Untargeted platform
We perform untargeted biomarker research using a high resolution Q-TOF (AB-Sciex 5600 Triple TOF MS) to monitor and compare thousands of analytes from biological samples. Compounds of interest from statistical analysis are identified using either database matching of MS/MS patterns, comparison to standards, or de-novo identification using ultra-high resolution analysis (Orbitrap Fusion, Thermo-Fisher Scientific)
Quantitative / Targeted metabolomics panels
We measure several hundreds of metabolites using absolute quantification, utilizing stable isotope dilution, 6-8 pt standard curves and 4 levels of QC analysis within each batch. Most quantitative assays are run on a triple quadrupole LC-MS/MS system (API-4500, AB Sciex). Panels include:
Intracellular energy metabolite panel: Primary metabolites and cofactors associated with energy production in the cell. Absolute quantification of nucleotides (e.g. ATP, ADP, AMP, GTP, GDP,...etc), sugar phosphates (glycolysis, pentose phosphate intermediates), coenzymes/cofactors (e.g. NADH, NAD+, acetyl-CoA, etc). This platform uses either cell culture or tissue as the matrix for analysis (compound list).
Lipidomics Panel: Phospholipids, fatty acids (un-, polyunsaturated), eicosanoids, acylcarnitines, and bile acids. Relative quantification of a variety of hydrophobic metabolite classes critical for metabolism, modulating inflammation, and signaling. Peaks are normalized to appropriate stable isotope internal standards for ideal inter-sample comparisons. Method was developed for plasma and serum samples, but is adaptable to all types of biological tissues that contain measurable levels of these metabolites (compound list). Lipid compound list can be customized for individual projects.
Global metabolite panel: (ideal for plasma, serum, urine, or even cells). These several hundred metabolites are found both intra- and extracellularly and include amino acids, organic acids, acylcarnitines, purines and pyrimidines, bile acids and more. These compounds are quantified within two separate runs to cover multiple ionization modes. This panel may be measured in conjuction with the UCSD Biochemical Genetics Lab (www.ucsdbglab.org)
Nearly 40 compounds quantified in single run, with a stable isotope included for each compound
Resolution of each phosphorylation state
ensures no interferences in metabolite quantitation